what is the order of a blood draw

It is based on CLSI Procedures for Collection of Diagnostic Blood Specimens by Venipuncture. The correct order of draw follows.

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Blue If using a butterfly draw a blue waste tube Black top o ESR CBC etc.

. Sodium Polyanethol Sulfanate AKA SPS Sodium Citrate N0 Additive Clot activator Heparin either sodium or lithium EthyleneDiamineTetraacetic Acid AKA EDTA Potassium Oxalate with Sodium Fluoride Color Yellow Blue Red SST Green Lavender Grey A Useful Order of Draw Mnemonic. As colour coding and tube additives may vary verify recommendations with local laboratories. This is because bacteria from the non-sterile tube stoppers can result in bacteria growth which will show in the test as a blood infection.

This is to prevent cross contamination by the tube additives that could lead to erroneous results. Shaking the tube vigorously may cause hemolysis of the blood separating of red blood cells. Last draw - additive tubes in this order.

Phlebotomy order of draw is the same for specimens collected by syringe tube holder or into tubes preevacuated at the time of collection. This is known as the Order of Draw. BLOOD COLLECTION TUBES THE ORDER OF DRAW Version 82018 072010.

CLSI Order of Draw CLSI order of draw is as follows. SPECIMEN LABELING REQUIREMENTS All specimens submitted to the laboratory must be individually labeled. Blood Cultures Aerobic followed by anaerobic - if insufficient blood for both culture bottles use aerobic bottle only.

Approved Standard Sixth Edition October 2007. Draw one green and one orange bottle for adults. First - blood culture bottle or tube yellow or yellow-black top Second - coagulation tube light blue top.

The recommended order of draw for plastic vacutainer tubes is. It appears that the only exception to this rule is in drawing micro-containers from. Incorrect order of draw Consequence.

How to Remember the Order of Draw Quickly And Forever The problem with most mnemonics for the blood draw order is that theyre boring. This order of draw should be followed whenever multiple tubes are drawn during a single venipuncture. Draw blood collection tubes in the correct order to avoid cross-contamination of additives between tubes.

Learning how to draw blood is essential to becoming a phlebotomist. Light Blue Top plasma. BD BACTEC Plus Aerobic medium 442192 Blue Cap and bottle BD BACTEC Lytic Anaerobic medium 442265 Purple Cap and bottle BD BACTEC Peds Plus medium 442194 Silver cap pink bottle Cat.

Cheese lovers shouldnt have a hard time with this list except for maybe limburger which is definitely an acquired taste. Blood culture tubes are usually at the top of the order of draw to avoid contamination of the blood collected. The following order of draw is the approved order as established by CLSI.

The Order of Draw is designed to eliminate the possibility of cross contamination that may result in erroneous results. SST Sodium citrate EDTA and Sodium fluoridepotassium oxalate B. Blood culture tube or bottle.

B rie B lood Culture L imburger L ight Blue R icotta R ed G orgonzola G reen L eyden L avender and G ouda G ray. Specimen labels may be ordered from Laboratory Services please call Client Services. All tubes must be gently inverted 8 10 times to provide thorough mixing of additives.

SST red-gray or gold top. Blood collection tubes must be drawn in a specific order to avoid cross-contamination of additives between tubes. And in order to do so one must learn and follow the proper steps to collecting blood - from gathering the right equipment to transporting the sample to the laboratory.

Sodium citrate Red SST Heparin EDTA and Sodium fluoridepotassium oxalate. Theres nothing interesting unusual or memorable about them. To avoid cross-contamination blood must be drawn and collected in tubes in a specific order.

The only problem you might have with this memory tool is lusting for your lunch. When collecting diagnostic blood specimens by venipuncture into evacuated tubes the order of draw is b ased on Clinical and Laboratory Standards Institutes CLSI standard H3-A6 from November 2007 as follows. I mean this is what youll typically see websites and books suggesting.

Sodium citrate tube eg blue closure Serum tubes including those with clot activator and gels eg red red-speckled gold closures. Boys for blood culture variable Love for light blue. Plain Red Sodium citrate and SST D.

Must be drawn first to. Sed Rate Westegren Sed Rate sion Fill tube to line. When drawing blood What is the order of draw.

Correct order of draw. ORDER OF DRAW If there is a concern regarding contamination by tissue fluids or thromboplastins then one may draw a non-additive tube first and then the light blue top tube. What is the CLSI ASPT correct order of draw when using a butterfly.

Blood Culture Bottles Used for bacterial and fungal cultures. Blood Culture Tubes or Vials. Seizure and death from potassium carrying over from EDTA into tube to be tested for K Medication errors when additives carry over into coag tubes falsely lengthening coagulation times and leading to unwarranted and life-threatening medication adjustments.

Coagulation Tubes Blue-Top Tubes Serum Tubes without Clot Activator or Gel Red-Top tubes Serum Tubes with Clot Activator or Gel Gold or Tiger Top Tubes Heparin Tubes Green-Top Tubes EDTA Tubes. 1 Blood cultures yellow SPS sterile 2 Light blue buffered sodium citrate tube 3. SST Sodium citrate Sodium fluoridepotassium oxalate and EDTA C.

Draw samples in the correct order. One yel-low for pediatrics TB Fungal collect yellow isolator tube PT APTT Coagula-tion Studies green NOTE. Order of Draw for Vacutainer Collection Tubes 1.

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